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FUELSTAT – The Science

About Fuelstat™ resinae 

The Fuelstat™ resinae test utilises lateral flow technology and highly specific antibodies to Hormoconis resinae (HR) grown in fuel.

 

 

The gold labelled antibody is immobilised in the Release Pad under the sample well.

The sample containing an unknown amount of HR is added to the sample well and this re-hydrates, allowing the reagents to flow up (wicking) the membrane towards the absorbant pad. Any large particles in the sample, which may block the reaction, are blocked by the filtering action of the pad. During the wicking the HR in the sample will bind to the specific antibodies.

As the liquid reaches the test line (T) any free gold-labelled antibodies will bind to the test line. This means if more HR is in the sample than the threshold engineered, there will be no antibodies to bid to the test line, no red line will appear, and this is a POSITIVE result.

 

 

If the amount of HR in the sample is lower than the threshold, there will be free anitbodies to bind to the Test Line, a red line will appear, and this is a NEGATIVE result.

The quantities of materials immobilised in the device are engineered to provide results at the different thresholds of HR in each of the test devices on the Test Paddle.

There is an inbuilt Control in the test and for all samples run, a line should appear against the C (Control) point in the viewing window. If no line appears the test has failed and should be repeated.

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